Impact of fungicide seed coating on rhizobia survival and nodulation of pea plants

2018

Research organisatons
Funding source

Trial details

Researcher(s) Matthew Denton
Thang Viet Lai
Judith Rathjen
Maarten Ryder
Year(s) 2018
Contributor SARDI Minnipa Agricultural Centre
Trial location(s) Minnipa, SA
Impact of fungicide seed coating on rhizobia survival and nodulation of pea plants locations
Aims

To determine the potential toxicity of the fungicide P-Pickel T (PPT) to rhizobia applied as a commercial inoculant (peat and freeze-dried) on field pea (R. leguminosarum, group F) in field conditions in a soil with a low rhizobial background.

Key messages
  • Nodulation was reduced when pea seeds were coated with P-Pickel T fungicide before inoculation.
  • Reductions in rhizobia numbers on fungicide-coated seeds occurred very quickly, within two hours, but the toxic effect of the fungicide continued after sowing.
  • Dry soil conditions are likely to have exacerbated fungicide toxicity to the rhizobia.
Lead research organisation South Australian Research and Development Institute
Host research organisation SARDI Minnipa Agricultural Centre
Trial funding source GRDC 9176500
Related program N/A
Acknowledgments

GRDC project 9176500 for providing funding for this research. Amanda Cook (SARDI, MAC) for advice on trial management and MAC staff for their technical assistance. Liz Farquharson (SARDI, Waite) for advice and sampling, and Bruce Heddle for providing land for the trial.

 

Drew E, Herridge D, Ballard R, O’Hara G, Deaker R, Denton M, Yates R, Gemell G, Hartley E, Phillips L, Seymour N, Howieson J and Ballard N,  ‘Inoculating Legumes: A practical guide’. GRDC, July 2012


Other trial partners University of Adelaide
Download the trial report to view additional trial information

Method

Crop type Grain Legume: Field peas
Treatment type(s)
  • Fungicide: Application Method
  • Fungicide: Timing
  • Fungicide: Type
Trial type Experimental
Trial design Randomised,Replicated,Blocked

Minnipa 2018

Sow rate or Target density estimated plant density of 54 plants per m2.
Sowing machinery

A field site with low background of field pea rhizobia was selected on Bruce Heddle’s property, near Minnipa Agricultural Centre. The trial was a completely randomised design with three replications. Pea cv. Oura seeds were coated with the label recommendations for PPT, and then inoculated with either a commercial freeze-dried or peat formulation, again at commercial rates. Seed was sown immediately (0 h) or stored at room temperature in the dark for 24 h before sowing. Plots with no inoculation (Nil) were also sown as controls. The plot sizes were 12 m x 1.8 m with 25 cm row spacing (6 rows), and an estimated plant density of 54 plants per m2.

 

Before sowing, rhizobial counts from the inoculated and PPT treated/untreated seeds were performed to determine if there were adequate numbers of rhizobia on seed. Seed samples (10 seeds) from all treatments (excluding Nil) were&

Sow date 30 June 2018
Harvest date Unknown
Plot size 12m x 1.8m
Plot replication 3
Plot randomisation completely randomised design
Download the trial report to view additional method/treatment information
Trial source data and summary not available
Check the trial report PDF for trial results.
Observed trial site soil information
Trial site soil testing
Not specified
Soil conditions
Trial site Soil texture
Minnipa, SA Red sandy loam
Derived trial site soil information
Australian Soil Classification Source: ASRIS
Trial site Soil order
Minnipa, SA Calcarosol
Soil Moisture Source: BOM/ANU
Average amount of water stored in the soil profile during the year, estimated by the OzWALD model-data fusion system.
Year Minnipa SA
2018 293.5mm
2017 323.8mm
2016 315.3mm
2015 289.3mm
2014 336.5mm
2013 290.8mm
2012 330.8mm
2011 383.4mm
2010 385.3mm
2009 391.3mm
2008 330.4mm
2007 331.2mm
2006 334.5mm
2005 288.0mm
2004 296.1mm
2003 293.2mm
2002 283.3mm
2001 343.9mm
2000 389.0mm
National soil grid Source: CSIRO/TERN
NOTE: National Soil Grid data is aggregated information for background information on the wider area
Actual soil values can vary significantly in a small area and the trial soil tests are the most relevant data where available

Soil properties

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Climate

Minnipa SA 2018


Observed climate information

Rainfall avg ann (mm) 325mm
Rainfall avg gsr (mm) 242mm
Rainfall trial total (mm) 244mm
Rainfall trial gsr (mm) 178mm

Derived climate information

Minnipa SA

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Some data on this site is sourced from the Bureau of Meteorology

SILO weather estimates sourced from https://www.longpaddock.qld.gov.au/silo/
Jeffrey, S.J., Carter, J.O., Moodie, K.B. and Beswick, A.R. (2001). Using spatial interpolation to construct a comprehensive archive of Australian climate data , Environmental Modelling and Software, Vol 16/4, pp 309-330. DOI: 10.1016/S1364-8152(01)00008-1.



Trial last modified: 03-01-2023 10:39am AEST