• A literature search was conducted of research into trace elements in crop production systems of south eastern Australia.
• References found in the "grey" literature (primarily printed material not available on-line) was summarised into key points and entered into the On Farm Trials database.
• All of these entries, plus those found from searching the international peer reviewed journal literature were entered into an Endnote database.
• The Endnote database is made available here, along with a text version for use by researchers.
• This activity was funded by the GRDC More Profit from Crop Nutrition program.
• Keywords: zinc, manganese, molybdenum, iron, copper, boron, trace elements, micronutrients.

From this trial:

• No crop response to the addition of Manganese. Plant tests not in the deficient range.

From the whole project:

• Current guidelines are still relevant and effective for TE management.
• Some current interpretations of soil tests are causing issues - the suggested levels to avoid deficiencies are much higher in some lab reports than is our experience from field trials. These are creating false impressions of the extent and severity of Zn and Cu deficiencies.
• No evidence of a major increase in the incidence or severity of TE deficiencies in the cropping zones.
• Fluid delivery of TEs at seeding is a valid management strategy but rates of the target TE need to be the same as used with compounded fertilisers.
• Generally, all sources of foliar TE were equally effective.

From this trial:

• No crop response to the addition of Manganese. Plant tests not in the deficient range.

From the whole project:

• Current guidelines are still relevant and effective for TE management.
• Some current interpretations of soil tests are causing issues - the suggested levels to avoid deficiencies are much higher in some lab reports than is our experience from field trials. These are creating false impressions of the extent and severity of Zn and Cu deficiencies.
• No evidence of a major increase in the incidence or severity of TE deficiencies in the cropping zones.
• Fluid delivery of TEs at seeding is a valid management strategy but rates of the target TE need to be the same as used with compounded fertilisers.
• Generally, all sources of foliar TE were equally effective.

• Current guideline of YEB values at tillering greater than 15mg Mn/kg indicative of adequate status still appear appropriate.
• No yield response recorded to application of Mn in different form or timing.

From this trial:

• No crop response to the addition of Manganese. Plant tests not in the deficient range.

From the whole project:

• Current guidelines are still relevant and effective for TE management.
• Some current interpretations of soil tests are causing issues - the suggested levels to avoid deficiencies are much higher in some lab reports than is our experience from field trials. These are creating false impressions of the extent and severity of Zn and Cu deficiencies.
• No evidence of a major increase in the incidence or severity of TE deficiencies in the cropping zones.
• Fluid delivery of TEs at seeding is a valid management strategy but rates of the target TE need to be the same as used with compounded fertilisers.
• Generally, all sources of foliar TE were equally effective.

From this trial:

• No change in crop performace with the addition of Manganese. As a foliar spray, sulphate was as effective as an EDTA chelate at increasing leaf manganese at Z30. Halving the recommended rate of sulphate decreased leaf manganese levels.

From the whole project:

• Current guidelines are still relevant and effective for TE management.
• Some current interpretations of soil tests are causing issues - the suggested levels to avoid deficiencies are much higher in some lab reports than is our experience from field trials. These are creating false impressions of the extent and severity of Zn and Cu deficiencies.
• No evidence of a major increase in the incidence or severity of TE deficiencies in the cropping zones.
• Fluid delivery of TEs at seeding is a valid management strategy but rates of the target TE need to be the same as used with compounded fertilisers.
• Generally, all sources of foliar TE were equally effective.

• Applying N at GS31 increased yield but no response to manganese was measured.

• There was a dry matter response measured at anthesis to all treatments with application of manganese but due to dry season this was not translated into yield increases.

• A diagnostic service is available to farmers and industry for diseased plant specimens.
• Samples of all leaf and aerial plant parts should be kept free of moisture and wrapped in paper not a plastic bag. Roots should be dug up carefully, preserving as much of the root system as possible and preferably kept damp. Samples should be sent to the following address:

SARDI Diagnostic Centre
Plant Research Centre
Hartley Grove
Urrbrae SA 5064

• Single foliar trace element applications may not be sufficient to correct deficiencies caused by spreading calcareous clays.
• Placement of copper, zinc and manganese below the incorporated clay layer appeared to partially address the problem.
• This was an unreplicated farmer demo so needs to be repeated with replication to improve interpretation of results.

• Treated seed treatment had a marginally higher yield (3.24t/ha versus 3.15), lower screenings, lower protein and higher income by $9/ha.
• Seed from control plots (untreated seed sown) had higher nutrient (Mn, Cu, Zn, P and S) concentrations.
• Coating seed does no harm but farmers need to judge what is ' best bang for their buck'.

• Subsoil nutrition placed at 20cm has provided a residual yield benefit in the second year after application at Edillilie.
• Yield increases were greater where trace elements were also applied.

• The +Zn treatment received 7 kg Zn/ha at sowing plus 2 foliar sprays of 0.33 kg Zn/ha.
• Generally, yield responses to Zn are greater in durum and bread wheat than in barley.
• The most inefficient lines of wheat and barley showed distinctive symptoms of Zn deficiency, such as reduced growth, pale leaves with chlorotic and necrotic lesions along the mid-vein.
• In 1999 a dry spring limited the yield response to Zn to about 0.5 t/ha, whereas, in 1998 yield increases up to 1 t/ha were measured.
• The disparity between the vegetative and the yield responses has tended to be greater in barley than in wheat.
• The wheat cultivars Goldmark and Kukri have low Zn efficiency, whereas the widely adapted, high yielding cultivars Trident, Krichauff and Worrakatta are Zn-efficient.
• Within barley, a number of Japanese breeding lines (eg SBWI-1) are very inefficient, but many current cultivars from SA as well as Arapiles (Vic.) and Stirling (WA) show a high level of efficiency.
• Pot assays for Zn efficiency have been developed and generally show similar rankings to the field tests.
• Wheat double haploid populations derived from the cross between Trident (efficient) and Songlen (inefficient) have been produced to develop molecular markers for Zn efficiency.

• The relative sensitivity to manganese deficiency is broadly : bread wheat (least), barley, durum wheat (most).
• The relative sensitivity to zinc deficiency is broadly: barley (least) bread wheat, durum wheat (most).
• With a very inefficient variety adding a micronutrient fertiliser may not be sufficient to overcome the deficiency.
• Special attention needs to be given to the micronutrient requirements of durum wheat especially where high rates of N and P fertilisers are used.

• At Wangary SA when barley seed with a high manganese content was sown there was an increase in yield beyond that achieved with managnese fertiliser.
• Seed produced from manganese deficient or marginal soils can have low manganese, post anthesis foliar sprays can increase grain manganese conentration and improve the growth of a crop sown from that seed.
• At Marion Bay SA the timing of manganese spraying during grain fill was not critical.
• Yields of barley grown from barley seed gathered from a number of different sources and sown at a Callington SA correlated very strongly with the concentrations of copper and zinc in the sown seed.

• Initial barley growth was increased 40-50% and final yield by 10% when using seed with greater nutrients, even on soils not associated with manganese deficiency.
• Crops can drop into manganese deficiency late in the season with symptoms being pale, floppy leaves.
• Sufficient concentraiotns are Copper 2-3 ppm in grain and 5ppm in leaf, and for zinc 15ppm in grain and 23-25 ppm in leaf.
• Zinc is so cheap thatbit is worthwhile treating than taking any risk, at Wudinna this year a headland area not receiving zinc died while the remainder of the crop which received zinc survived the water stress.
• Trace element deficiencies seem to beexacerbated in dry years.

• Correction of zinc deficiency in a barley crop increased grian yield and reduced Rhizoctonia bare patch.
• In glasshouse experiments, increasing wheat shoot zinc to 20 mg/kg dry matter strongly suppressed damage to roots by the Rhizoctonia fungus.
• Experiments also showed that the zinc was ot toxic to the fungus but instead improved root growth.

• A critical level of zinc is required in the soil before roots will either grow into it or function effectively.
•  It is likely that this requirement is frequently not met in deep sandy in fertile soils.
• This paper presents arguments for breeding cereal varieties with root system is better able to mobilise zink from soil sources of low availability.
• Significant genetic variation for this character is described for wheat, barley and oats.
• Linkage to other efficiency traits for example manganese, is poor suggesting independent mechanisms and genetic control not linked to gross root system geometry.
• Zinc efficiency traits for sandy and clay soils are appear be genetically different.
•  Zinc inefficient genotypes absorb more zinc from deficient soils.

• Mn enriched fertilisers at seeding and foliar sprays were effective at correcting Mn deficiency in barley.

• A series of field experiments was conducted on the Eyre Peninsula to examine the effects of herbicides on the nutrition of treated crops.
• Experiments investigating the effect of metsulfuron-methyl and chlorsulfuron on crop performance are reported here.
• The parameters measured were shoot growth, tissue nutrient concentration and grain yield.
• Application of metsulfuron-methyl to manganese-deficient barley and chlorsulfuron to zinc-deficient wheat crops caused yield penalties by intensifying the respective deficiencies.
• Yields were not affected when the herbicides were applied to plots which were trace element adequate.
• The consequences are that if adequate fertiliser regimes are not employed in the field, yield benefits due to weed control may be eroded by losses due to aggravated micronutrient deficiencies.

• The first leaf should not be used to diagnose manganese deficiency in barley as it has a higher concentration than the youngest expanded blade.
• Visual symptoms of manganese deficiency begin on the youngest leaves but necrosis develops slowly.
• There was no difference in manganese concentration between the youngest expanded blade on the main stem or tillers so either could be used.
• Chlorophyll a fluorescence was strongly correlated with manganese concentration and a useful tool for identifying manganese deficiency in barley.

• Several barley and wheat cultivars displaying a broad range of tolerance to boron toxicity were cultured in solution culture at boron concentrations from adequate to toxic.
• Tolerant cultivars developed fewer symptoms, grew better and absorbed less boron than sensitive varieties.
• The supply of boron had no consistent effects on the tissue concentration of macronutrients (K, S, Mg, P, Ca) and trace elements (Cu, zinc, Mn, Fe).
• There appeared to be no interaction between boron and other elements.
• It was concluded that differences between cultivars in tolerance related to the amount of boron absorbed.

• Severity of root rot of barley and wheat in farmers fields caused by Rhizoctonia was inversely correlated with crop Zn status.
• Zn was the only nutrient correlated with the severity of Rhizoctonia.
• Zn deficient plants were more susceptible to Rhizoctonia.
• 2.5 kg Zn/ha halved the area suffering from bare patch.

• Barley crops showing foliar symptoms of a disorder recognised as boron toxicity were analysed for boron and other nutrients.
• Samples were obtained from sites throughout the cropping districts of South Australia.
• In plants with characteristic foliar symptoms, the concentration of boron alone was sufficient to account for the crop disorder.
• The mean concentration in affected plants was 151 mg/kg and in control plants 23 mg/kg.
• Slight accumulations of sodium and chloride were associated with the disorder, along with slightly lower calcium concentrations.
• The nutrient balance was consistent with association of the toxicity with sodic and saline soils.
• Severe boron toxicity is widespread in barley crops in South Australia.

• A factorial pot experiment involving four rates of soil-applied Mn (0, 0.75, 7.5 and 75 mg Mn/pot), four levels of CCN (0, 93, 186 and 372 second stage juveniles per pot) and 12 replicates of one Hordeum vulgare cv. 'Clipper' plant per pot was carried out.
• Four replicates were harvested 28, 73 and 192 days after sowing and each plant was assessed at harvest for growth, Mn content and level of CCN infection • Several plant growth parameters were measured non-destructively during growth.
• Resistance (ability of the pathogen to reproduce on the host) was not influenced by Mn nutrition of the host and equal numbers of nematodes invaded and developed to maturity on all plants despite Mn treatments resulting in plant Mn levels which ranged from severely deficient (0 Mn rate) and deficient (low Mn rate) to adequate (two highest Mn rates).
• However, tolerance (endurance of disease without severe losses in yield or quality) was considerably reduced by the combination of low Mn supply and higher levels of CCN.
• Plant height was reduced by increasing CCN numbers only at the two lowest Mn rates.
• These trends were typical of those observed in all plant growth parameters measured in the final two harvests including dry and fresh weight of roots and tops and total dry weight of grain produced per plant. Maturity was delayed in the severely affected plants.

• Application of Mn or Mn+nitrogen increased barley yield.
• There may have been some residual value of the applied Mn.

• Higher seed manganese content increase growth and yield of barley on manganese deficient soil.

• Mn deficiency reduces the tolerance of barley to infection by CCN (ie increases the damage caused).
• Mn deficiency does not change the resistance of barley to infection by CCN (ie multiplication of CCN is the same).

• Both higher seed manganese concentration or seed coating with MnSO4 were found to increase yield of barley on manganese deficient soil

• One hundred barley lines from a world collection were grown in plant breeders' plots (4 rows x 5 m) on a severely manganese-deficient soil.
• Only 10% of the lines showed good tolerance of manganese-deficient conditions, which were severe enough to kill many of the entries in the tillering stage.
• Extreme sensitivity to manganese deficiency was shown by all progeny of CI3576, an introduction from Egypt, which has conferred considerable yield advantage and disease resistance on its progeny under more highly fertile conditions.
• In all, there were 20 lines from CI3576, including Corvette, and Galleon, a newly released high-yielding feed barley.
• At present, Galleon is not recommended for calcareous sands, pending further agronomic work on its manganese fertilizer requirements.
• The established variety Clipper and its relatives, and Forrest, were also sensitive to manganese deficiency. The tolerant lines fell into three genetically related groups, all of which had pedigrees going back to traditional English land races or their derivatives.

• Wheat, barley, triticale, canary seed, sorghum, sunflowers, millet and panicum were grown in a copper deficient clay soil in pots in the glasshouse.
• For rates of copper from 0 to 6 mg per pot for winter crops, and the same plus 60 mg per pot applied to the summer crops.
• A rate of 6 mg per pot was not sufficient to maximise yield from the winter crops but some are crops produced maximum yields when 60 mg per pot was applied.
• Significant differences intolerance to copper deficiency were apparent between varieties within crops.
• Only the summer crops in triticale produce grain when nearly all the lowest rates of copper were applied.

• A series of tables listing concentrations of nutrients, including manganese, measured in the grain of a range of crops.

• 67% of divalent manganese incubated on calcareous sands form Warooka was immobilised within 167 hours by chemical and biological processes.
• In field experiments over seven years manganese up to 16 kg/ha drilled with the barley crop did not prevent manganese deficiency in crops.
• However, manganese applicaiotn increased crop growth, delayed deficiency symptoms and increased grian yield by an average of 61%.
• The optimum applicaiotn at seeding was 6 kg/ha manganese (25 kg/ha nanganese sulphate).
• Three foliar sprays of manganese did not completely correct manganese deficiency.
•  The best results were obtained by applying 6 kg manganese at sowing followed by three foliar sprays.